Welcome to the homepage of the Single Molecule Localization Microscopy Symposium! This edition of SMLMS aims to bring together scientists in the field of single-molecule localization microscopy and super-resolution imaging technologies, covering topics such as new imaging methods, fluorescent probes, labeling strategies, biological applications, and data analysis approaches. Vienna has a vibrant imaging community and is a pleasant destination to visit. The Scientific Committee includes Mike Heilemann from Goethe University Frankfurt, Germany, Helge Ewers from Freie Universität Berlin, Germany, Aleksandra Radenovic and Suliana Manley from EPFL, Switzerland.